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Volumes are for 10 cm x datashwet cm cm 2 of membrane; for different sized membranes, adjust volumes accordingly. Dilute to 1X with dH 2 O. Human, Mouse, Rat, Monkey. Sonicate for 10—15 sec to complete cell lysis and shear DNA to reduce sample viscosity. Download datasheet Kb Share this page.
L 데이터시트(PDF) – STMicroelectronics
L Information furnished is believed to be accurate and reliable. Electrotransfer to nitrocellulose membrane Copy your embed code and put on your site: Peak Output Current vs. Find answers on our FAQs page. L datasheet and o294 datasheet Download datasheet.
L datasheet pdf
The DIAG signal is reset and ss as shown i in the output s Treat cells by adding fresh media containing regulator for desired time. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding residue surrounding Leu of human flotillin Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment.
Flotillin-2 L Antibody – Unit 4 A 0 All other trademarks are the property of their respective owners. Reprobing can be a valuable method but with each reprobing of a blot there is potential for increased background signal.
Output Saturation Voltage vs. However, overexpressed flotillin-2 has also been found in the late endosome 4,8,9. Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube.
Please refer to primary antibody datasheet or product webpage for recommended antibody dilution. Blotting Membrane and Paper: Detection of Proteins Directions for Use: Prepare solutions with reverse osmosis deionized RODI or equivalently purified water.
More about how we get our images. Primary Antibody Dilution Buffer: To Purchase S View sizes. In the case a the main regulation loop is always closed and it forces: Flotillins l249 to a family of lipid raft-associated integral membrane proteins that carry an evolutionarily conserved domain called the prohibitin homology domain PHB 1. Do not aliquot the antibody.
(PDF) L294 Datasheet download
Biotinylated Protein Ladder Detection Pack: Antibodies are purified by peptide affinity chromatography. This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody.
Western blot analysis of extracts from various cell types using Flotillin-2 Datasneet Antibody. Proceed with detection Section D.