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A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes.

Identification of two distinct regulatory regions adjacent to the human beta-interferon gene. Human beta-interferon gene expression is regulated by an inducible enhancer element. Interaction of positive and negative regulatory proteins in the galactose regulon of yeast. Short and long range activation by the SV40 enhancer. Reversible silencing of enhancers by sequences derived iirf the human IFN-alpha promoter.

A rapid and sensitive assay system for bacterial gpt activity in irv mammalian cells. IRF-1 may also be involved in silencing the function of the SV40 enhancer juxtaposed to the regulatory elements in uninduced cells. Heat shock factor is regulated differently in yeast and HeLa cells.

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Open in a separate window. BK viral enhancer element and a human cellular homolog. National Center for Biotechnology InformationU. Delimitation and properties of DNA sequences required for the regulated expression of human interferon-beta gene.

Abstract Transcription of the human interferon-beta IFN-beta gene is induced by a variety of agents such as viruses, dsRNA and some cytokines. Images in this article Image on p. Tissue-specific expression of the human growth hormone gene is conferred in part by the binding of a irg trans-acting factor.

The 5′-flanking sequence of human interferon-beta 1 gene is responsible for viral induction of transcription.

In this study, we describe a nuclear factor, termed interferon regulatory factor-1 IRF-1that is involved in the iff of IFN-beta and possibly other genes. Regulation of cytokine gene expression.

Intracytoplasmic phosphorylation sites of Tac antigen p55 are not essential for the conformation, function, and regulation of the human interleukin 2 receptor.

High efficiency DNA-mediated transformation of primate cells. Associated Data Supplementary Materials.

Human embryonic lung cells HEL-R66 are of monkey origin. Support Center Support Center. The human beta-interferon gene ird is under negative control. Changes of chromatin conformation around mouse interferon-beta gene associated with induction of interferon synthesis. Biochemistry of interferons and their actions. This article has been cited by other articles in PMC.

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Structure of a chromosomal gene for human interferon beta. Establishment of a permanent cell line HEL-R66 from human embryonic lung cells with high susceptibility to viruses. Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase. Sequencing end-labeled DNA with base-specific chemical cleavages.

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Controlled transcription of a human alpha-interferon gene introduced into mouse L cells. Our data suggest that IRF-1 participates in the transient formation of an induction-specific complex es with the regulatory elements. A nucleotide promoter segment from an IFN-alpha gene renders an unrelated promoter inducible by virus.

Affinity purification of sequence-specific DNA binding proteins. Please review our privacy policy. Transcription of the human interferon-beta IFN-beta gene is induced by a variety of agents such as viruses, dsRNA and some cytokines. Author information Copyright and License information Disclaimer. Regulation of gene expression by interferons: Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.