Spodoptera litura reared on different host plants and assayed against SlNPV KETAHANAN SERANGAN HAMA ULAT GRAYAK (SPODOPTERA LITURA F.). Hama Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae) merupakan hama penting pada tanaman bawang di Indonesia. Pengendalian hama ini dengan. Potensi Jamur Entomopatogen Untuk Mengendalikan Hama Ulat Grayak ( Spodoptera litura) Fabricius (Lepidoptera: Noctuidae) Pada Tanaman Tembakau di.

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Directory of Open Access Journals Sweden. Full Text Available The use of chemical insecticide in crop protection around the world has resulted in disturbances of the environment. Therefore, it is necessary to develop for environmentally friendly insect pest management techniques such as the activity of liquid smoke made from tobacco stem waste as an insecticide to Spodoptera litura.

Activity of liquid smoke of tobacco stem waste was carried out at the laboratory condition. The results showed that the application of liquid smoke by using the spraying method direct method showed better results compared to the results of feeding method spkdoptera method. The sub lethal concentration did not cause mortality spodopteraa S.

Liquid smoke of tobacco stem waste has activity as an insecticide to S. Maka teknik pengendalian hama yang ramah lingkungan perlu dikembangkan, misalnya aktivitas asap cair limbah batang tembakau sebagai insektisida pada Spodoptera litura.

Aktivitas asap cair limbah batang tembakau dilakukan pada kondisi laboratorium. Hasil gama menunjukkan bahwa aplikasi asap cair secara langsung metode semprot lebih efektif daripada tidak langsung metode celup pakan. Konsentrasi subletal tidak menyebabkan kematian Apodoptera. Asap cair limbah batang tembakau bersifat insektisida terhadap larva S. Nutrisi diperlukan oleh serangga untuk pertumbuhan dan perkembangannya, sebagai sumber energi, perbaikan jaringan dan reproduksi. Kebutuhan nutrisi serangga pada umumnya berupa: Tujuan praktikum ini adalah untuk mengetahui efisiensi pemanfaatan pakan daun brokoli, daunt alas, daun bawang, daun kangkung spodopterx daun kacang panjang oleh larva S.

spodoptera litura larvae: Topics by

Bahan-bahannya adalah daun brokoli, daun bawang, daun kangkung, daun kacang panjang, dan daun bawang, Larva Spodoptera litura dan Larva C. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap RAL terdiri dari 6 perlakuan jenis pakan dan 10 ulangan. Histological study of SlNPV infection on body weight and peritrophic membrane damage of Spodoptera litura larvae. The method was used Probit analysis, and based on LD 50 the virus was infected to know body weight and post infection damage.

Spodopterra histological material was prepared by using parafin method litrua fixation with Bouin Solution, then slice into 7 um and colored with Spodoptear. The descriptive obsevation on structural intact of peritrophic membrane histology caused by SlNPV infection shows a tendency to decrease, while in control, there was no damage at all.

The longer the exposition of virion wpodoptera the midgut lumen the more damage on peritrophic membrane occurred. The severest hsma occurred 96 hour after infection. The result prove that haNPV virion can destroy hystological structure of midgut. Using biotransformation as a biocatalytic process has the advantage of being able to proceed under mild conditions and with high regio- and enantioselectivity. The metabolism of substrate 2 generated three enantiomers of the above metabolites, designated as torespectively.

Diversity and biological activity of nucleopolyhedroviruses of the leafworm Spodoptera litura. Increased resistance of emerging hhama leafworm Spodoptera litura in Pakistan and elsewhere to chemical insecticides calls for an alternative method of control. Isolates of nucleopolyhedrovirus NPVs of S. Full Text Available Azadirachtin is an efficient and broad-spectrum botanical insecticide against more than kinds of agricultural pests with the effects of mortality, antifeedant and growth regulation.

Real-time quantitative polymerase chain reaction RT-qPCR could be one of the powerful tools to analyze the gene expression level and investigate the mechanism of azadirachtin at transcriptional level, however, the hams reference genes are needed to normalize the expression profiling of target genes. In this present study, the fragments of eight candidate reference genes were cloned and identified from the pest Spodoptera litura.


In addition, the expression stability of spodiptera genes in different samples from larvae of control and azadirachtin treatments were evaluated spovoptera the computational dpodoptera of NormFinder, BestKeeper, Delta CT, geNorm, and RefFinder. According to our results, two of the reference genes should be the optimal number for RT-qPCR analysis. Furthermore, the best reference genes for different samples were showed as followed: Azadirachtin is an efficient and broad-spectrum botanical insecticide against more than kinds of agricultural pests with the effects of mortality, antifeedant and growth regulation.

Development and validation of real-time PCR tests for the identification of four Spodoptera species: Spodoptera eridania, Spodoptera frugiperda, Spodoptera wpodoptera, and Spodoptera litura Lepidoptera: The genus Spodoptera comprises 31 species, 4 of which are listed as quarantine pests for the European Union: In international trade, the earlier life stages eggs and larvae are being intercepted at point of inspection most frequently, challenging the possibilities of morphological identification.

To realize a rapid and reliable identification ha,a all stages, liturz developed and validated four simplex real-time polymerase chain reaction identification tests based on the mitochondrial cytochrome b gene using dual-labeled hydrolysis probes.

Method validation on dilutions of extracted DNA of the target organisms showed that low levels of template up to 0. No cross-reactivity was observed with 14 nontarget Spodoptera and 5 non- Spodoptera species in the specific Spodoptera tests.

The new Spodoptera tests have proven to be suitable tools for routine identification of all life stages of S. Characterization of A Baculovirus of Spodoptera litura Lepidoptera: Noctuidae Isolated from Yogyakarta. Full Text Available A Baculovirus has liura isolated from cadaver of larvae of Spodoptera lituraa Noctuidae of agricultural pest, importance due to its wide-range hosts and the damage to their respective host.

Phase contrast light microscopy observation from infected larvae showed that the fat body, hemocyte cells, and cells surrounding the trachea or tracheolus were the most tissue invaded by polyhedra. Transmission electron microscopy analysis of the occlusion body purified from diseased larva showed that the baculovirus envelope containing multiple nucleocapsid. Digestion of viral DNA with three restriction enzymes showed that the genome pattern of baculovirus isolated from Bantul were close to SpliNPV isolated from Japan and those of Spodoptera littoralis and quite distinct from those isolated from Southeast Asia region.

Bioassay test performed on first to fifth instar larvae showed that the virus effectively control the young larvaebut showed some level of resistance against older larvae of Spodoptera litura.

Full Text Available Hwma spp. The purpose of this research spodoptrra to study the pathogenicity of Metarhizium spp. The isolates were collected from rhizosphere of different crops i.

The results showed that there was effect of all isolates on egg mortality. First instar larvae was also died 3 days after eclosion.

The maximum mortality of first instar larvae was Full Text Available Spodoptera litura Fabricius is the most serious pest of many cultivated and non-cultivated crops and it developed resistance against many synthetic pesticides. The impact of a Compositae annual weed, Synedrella nodifl ora Gaertn. The LD50 results revealed that methanol extract is the most toxic to the pest followed by benzene and chloroform, petroleum ether — C and water.

Qualitative phytochemical analysis has also been performed. Host plants of leaf worm, Spodoptera litura Fabricius Lepidoptera: Full Text Available Spodoptera litura is a notorious leaf feeding insect pest of more than one hundred plants around the Asia-Pacific region. Host plant survey for two years from three different locations in cotton belt revealed 27 plant species as host plants of S. Major host plants on which it thrived for maximum period were Gossypium hirsutum L. Eggs were also collected from tree plants but larvae did not complete their development.


Full Text Available Eriborus argenteopilosus is the most important parasitoid attacking cabbage pest Crocidolomia pavonana in Indonesia. Previous studies proved that parasitoid encapsulation was found to be an important factor limiting the effectiveness of the parasitoid in controlling pest population in the field. Sincewe have conducted series studies to investigate encapsulation mechanism developed by hosts against parasitoid, responses of parasitoid toward encapsulation ability and to determine factors that may help parasitoid avoid encapsulation.

Parasitoid responses were examined on two different hosts C. Our findings showed that parasitization level was found to be high both on C. Encapsulation occurred to be high in all larva stages of C. We recorded that encapsulation in the larval body of C.

Melanization was only recorded in encapsulated parasitoid inside larva body of C. We recorded that encapsulation increased blood cell number of both larvae C. Encapsulation may affect development of immature parasitoid. Our investigation also proved that superparasitism may help parasitoid avoid encapsulation.


Characterization of putative virulence factors of Serratia marcescens strain SEN for pathogenesis in Spodoptera litura. Two Serratia marcescens strains, SEN and ICC-4, isolated from diseased insect spodopterra were observed to differ considerably in their virulence towards Spodoptera litura. The present study was aimed to characterize the possible virulence factors present in the virulent Serratia marcescens strain SEN.

Although, all the three toxin genes shlA, phlA and swr could be detected in both the S. Proliferation of the S. Peritrophic membrane treated with broth culture of the S. The peritrophic membrane of larvae fed on diet treated with the virulent strain showed considerable damage while the peritrophic membrane of larvae fed on diet treated with spodooptera non-virulent strain showed no damage. This is the first report documenting the fate of ingested S.

Growth, development, reproductive competence and adult behaviour of Spodoptera litura Lepidoptera: Noctuidae reared on different diets. Spodoptera litura was reared on natural food castor leaves, Ricinus communis and on a several semi-synthetic diets using quasi mass rearing techniques.

The effect of the different diets and rearing regimes on S.

Spodoptera litura reared from a modified chickpea-based diet provided the greatest growth index and index of adequacy. These studies were conducted as a prerequisite for the evaluation of F 1 sterility technique. Response of the common cutworm Spodoptera litura to zinc stress: Zn accumulation, metallothionein and cell ultrastructure of the midgut.

By exposing the common cutworm Spodoptera litura Fabricius larvae to a range of Zinc Zn stress, we investigated the effects of dietary Zn on Zn accumulation, metallothionein MTand on the ultrastructure of the midgut.

There was a significant dose—response relationship between the Zn accumulations in the midgut of the larvae and the Zn concentrations in the diet. TEM showed that numerous electron-dense granules EDGs and vacuoles appeared in the cytoplasm of the midgut cells, their number and size being closely correlated with the Zn accumulations in the midgut.

Moreover, the nuclei were strongly influenced by Zn stress, evidenced by chromatin condensation and irregular nuclear membranes.

When the herbivorous insect Spodoptera litura Fabricius larvae were fed on the artificial diet with pitura concentrations of Zn. Azadirachtin, the environmentally friendly botanical pesticide, has been used as an antifeedant and pest growth regulator in integrated pest management for decades.