CISSUS POPULNEA PDF

Some Nigerian males across various geographic and socioeconomic strata employ the use of herbals such as Cissus populnea extracts. Cissus populnea (Guill & Perr): A Study of the Aqueous Extract as Potential Spermatogenic Enhancers in Male Wistar Rats. Anthony B. Ojekale, Oladipupo A . Cissus populnea Guill. & Perr. [family VITACEAE]. Herbarium. Royal Botanic Gardens, Kew (K). Collection. Useful Plants of West Tropical Africa. Resource Type.

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Sertoli cells support all cells of the germ series as they pass through the various stages of development from primordial spermatogonia to fully differentiated free spermatozoa. The ability of the water extractible phytochemicals from stem bark of C. Populnda of Total Phenolic Content The total phenolic content was determined using the method reported by Singleton et al.

The vitamin C content of the plant is higher than that of related spices such as basil, bird pepper, black pepper, cinnamon, nutmeg, oregano, parsley and rosemary [ 33 ], and some commonly consumed green leafy vegetables in Nigeria [ 2526 ], green and pouplnea pepper, and some commonly consumed and underutilized tropical legumes [ 34 ].

The total flavonoid content of the sample was determined using a slightly modified method reported by Meda et al. A comparison of semen qualities between and The prevention of the chain initiation step by scavenging various reactive species such as free radicals is considered to be an important antioxidant mode of action [ 41 ].

Infertility is a major clinical problem, affecting people medically and psychosocially.

Anthraquinones of Cissus Populnea Guill & Perr (Amplidaceae)

The powdered leaves of Senna 5g was extracted as described for Cissus populnea Friedrich and Bailer, ; Brain and Turner, The stem bark of Cissus populnea was purchased from a traditional herbal practitioner in Lagos state and authenticated at the herbarium of the University of Lagos.

The animals were sacrificed at the end of 64 days under ketamine anesthesia. Significance was accepted at. However, the consumption of foods rich in antioxidant phytochemicals may help fight degenerative diseases caused by oxidative stress by improving body’s antioxidant status. The plant has also been reported to increase cell proliferation in Sertoli cell lines in vitro [ 37 ].

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However, the aqueous extract of stem bark of C. Sperm morphology was assessed from slides prepared with one drop from the original sperm suspension. The Federal Department of Forestry; The plant is also used in the treatment of sore breast, indigestion, veneral diseases, intestinal parasites, oedema and eye problems.

Its extracts have been credited with antibacterial properties [ 7 ], as an antitrypanosomal plant and a source of gum powder [ 8 ] and as a component of a herbal antisickling Nigerian formula [ 9 ].

In Benin Republic, it is used for its diuretic properties while in Ghana it is used as a postharvest ethnobotanical protectant [ 10 ]. Archives of Applied Science Research, The rats were decapitated under mild diethyl ether anaesthesia, and the testes tissue were rapidly dissected and placed on ice and weighed.

Journal of Endocrinology, View at Google Scholar T. Veiw figure Figures index. Furthermore, the incubation of the testes tissue homogenate in the presence of FeSO 4 and SNP, respectively, caused a significant increase in the malondialdehyde MDA contents of the testes.

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Izzo, The aphrodisiac and adaptogenic properties of ginseng. The high vitamin C content of the plant will definitely contribute additively or synergistically to the observed antioxidant and medicinal properties of the plant. The study protocol was approved by the Institutional Ethical Committee. Cisus rats were allowed to assess food and water ad libitum. Foods of plant origin usually contain natural antioxidants that can scavenge free radicals cissu 6 ].

Likewise, the incubation of rat’s testes tissue homogenates in presence of sodium nitroprusside also caused a significant increase in the rat’s testes malondialdehyde MDA content as shown in Figure 1 b ; however, the extract inhibited MDA production content in the tissue in a dose-dependent 0—0. Materials and Methods 2. The effects were also compared with Addyzoa a commercially purchased fertility enhancing polyherbal formulation.

Appropriate dilutions of the extract were oxidized with 2. McGuinness, Development of postnatal gonocytes in vivo and in vitro, in Function of somatic cells in the testis. However, the aqueous extract of the stem bark of C.

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West African Plants – A Photo Guide – Cissus populnea Guill. & Perr.

The percentage content of anthraquinones of the stem bark of Cissus populnea is 1. Traditional Complementary and Alternative Medicines Woody Plants of Ghana. This study assessed the spermatogenic effects of aqueous extract of Cissus populnea stem bark.

The Practical Evaluation of Phytopharmaceuticals. Interstitium and visible Leydig cells are normal. Determination of Vitamin C Content Vitamin C content of the sample was determined using the method of Benderitter et al.

Extraction is based on the fact that the free anthraquinones are soluble in non polar solvents while the glycosides are soluble in polar solvents. The plant was identified on the field cissue description given in the monographs Irvine,Hutchinson and Dalziel, Sperm count was determined using the improved Neubauer haemocytometer.

Evidence has dissus that these damaging events are caused by free radicals [ 1 ]. Histology This followed cissux procedure as described [ 45 ]. There is a global observed decline in male fertility over the years consequent on various factors affecting spermatogenesis. Data were analysed with SPSS DPPH radical scavenging ability of aqueous extract of stem bark of Cissus populnea. The counting chamber was charged from this suspension carefully to avoid sperm accumulating in its trough.

Animals The procured Wistar rats were housed in well ventilated cages, kept in the University animal house and allowed to acclimatize in their new environment for 7 days.

The epididymis was used for estimation of sperm parameters. The tendencies of electron or hydrogen donation are critical factors in characterizing antioxidant activity that involves free radical scavenging [ 43 ].

Therefore, the higher reducing ability of Cissus populnea extract may have contributed to the higher protective effect observed.