CHARDAKOV METHOD PDF

2) In “Chardakov method for water potential measurement” experiment, you find that M solution has not been changed by water loss or absorption by the. Chardakov Technique. Plant Physiology. UNI. Incubating tissue in solution. Pre- incubation. Incubation. Post-incubation. Save for later measurement. Did solution change concentration? Solutes stay the same If water left or entered tissue –Water also entered or left solution –Solution becomes more dilute or.

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What is the water potential of potato tissue? This increases the tissue weight and may lead to small errors. Response of drops float, sink, hover when placed in solutions in which potato cores have been incubated.

Chardakov method

Change in Weight final – initial g. Work quickly to minimize chardakkv and keep the tissue wrapped in a moist towel. Dispense 10 mL of water or a sucrose solution 0. Use a cork borer to prepare at least 27 uniform tissue samples charfakov the potato. OK Any substance that can change shape or chsrdakov easily. This is due to incipient plasmolysis that occurs at low solution water potential. The Chardokov method provides a quick means to determine plant tissue water potentials.

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Put two or preferably three potato cores in each solution water or sucrose. Slowly release a drop of the methylene blue solution from the pipette and note whether the drop of the dye sinks, chardkov, or floats to the surface in this solution and subjectively estimate whether it does so rapidly or slowly. Feedback Privacy Policy Feedback. The water potential measured by this technique should be the same as that obtained the Chardakov method.

Examine the cores as you weigh them. Determine the approximate sucrose concentration for which there is no net change in density after tissue incubation i.

Change in weight of potato cores incubated in sucrose solutions. My presentations Profile Feedback Log out.

Chardakov method Essay – Free Papers and Essays Examples

Then, the percent change in weight of the tissue is plotted versus solution concentration or osmotic potential. Do the cores show various degrees of turgor? Be sure not to include any fragments of the skin. Which is more accurate?

In contrast to the Chardakov method which analyzes changes in solution density after incubation, this technique monitors tissue weight changes. Does it correspond to the value obtained by the Chardakov technique? We think you have liked this presentation. At this point, the water potential of the solution equals the water potential of the potato cores. Chardaov the cores for at least 1. Cytology Osmosis and Water relations.

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Share buttons are a little bit lower. Cut them to the same length ca.

If necessary, add more of the appropriate solution to completely submerge the cores but the final volume in each tube must be the same. Registration Forgot your password?

Hypertonic Concentration with higher solute concentration and less water concentration Hypotonic lower solute concentration and more water concentration. A measure of how much material is packed into a unit volume of the material The fewer. Incubating tissue in solution Pre-incubationIncubation Post-incubation Save for later measurement.