Aberrant splicing can result in deleterious consequences for the organism. . of 5 ′Bcl-x AS on inducing apoptosis (Mercatante and Kole, unpublished data). Antisense RNA (asRNA), also referred to as antisense transcript, natural antisense transcript . The asRNA that is responsible for this silencing effect is antisense non-coding RNA in the INK . Kole R, Krainer AR, Altman S (January ). Cellular Delivery and Antisense Effects of Peptide Nucleic Acid Conjugated to .. S. Fucharoen, H. M. Moulton, M. H. Nelson, N. Maeda, O. Smithies, R. Kole.

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In this approach, antisense activity of each oligomer was directly proportional to up-regulation of the EGFP reporter.

This provided a positive, quantitative readout for sequence-specific antisense effects of the oligomers in the nuclei of effcts cells.

Antisense RNA – Wikipedia

Nuclear localization of fluorescent labeled oligomers confirmed validity of the functional assay. The effects of the PNA oligomers were observed to be dependent on the number of l -lysine Lys residues at the C-terminus. The experiments suggest that the PNA containing Lys was taken up by a mechanism similar to that of cell-penetrating homeodomain proteins and that the Lys tail enhanced intracellular accumulation of PNA oligomer without affecting its ability to reach and hybridize to the target sequence.


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Nuclear antisense effects of neutral, anionic and cationic oligonucleotide analogs

Nuclear antisense effects of neutral, anionic and cationic oligonucleotide analogs Peter Sazani. Add comment Close comment form modal. I agree to the terms and conditions.

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Antisense RNA

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Receive exclusive offers and updates from Oxford Academic. Anti-tumor activity of splice-switching oligonucleotides. Expanding the action of duplex RNAs into the nucleus: Subnanomolar antisense activity of phosphonate-peptide nucleic acid PNA conjugates delivered by cationic lipids to HeLa cells.


Related articles in Web of Science Google Scholar. Pridopidine stabilizes mushroom spines in mouse models of Alzheimer’s disease by acting on antisnse sigma-1 receptor.

RNA splicing efects in genomic medicine. Changes in prolactin receptor homodimer availability may cause late feathering in chickens. Citing articles via Web of Science H-NS uses an autoinhibitory conformational switch for environment-controlled gene silencing.